human primary type ii alveolar epithelial cells (aecs)  (Procell Inc)

Journal: Respiratory Research

Article Title: Mesenchymal stem cells promote alveolar epithelial cell wound repair in vitro through distinct migratory and paracrine mechanisms

doi: 10.1186/1465-9921-14-9

Figure Lengend Snippet: Characterisation of hMSC and alveolar A549 cells. ( A ) Human type II alveolar epithelial cell line, A549 cells were positive for type II AEC marker pro SP-C. Phase contrast image is taken from an independent field to the pro SP-C and pro SP-C/DAPI field. ( B ) hMSCs isolated from human bone marrow aspirates were positive for mesenchymal stem cell markers CD44, STRO-1, CD90 and CD146; and negative for haematopoietic markers CD14 and CD19. ( C ) hMSC tri-lineage differentiation: osteogenesis, adipogenesis and chondrogenesis were confirmed by cytochemical/immunocytochemistry staining with Alizarin Red/anti-Osteocalcin, Oil Red O/anti-FABP-4 and Alcian Blue/anti-Aggrecan respectively. Scale bars, 100 μm.

Article Snippet: Human type II alveolar epithelial cell (AEC) line, A549 cells were purchased from ATCC, Rockville, USA and maintained in a continuous culture in DMEM supplemented with 10% FBS, 1% L-glutamine and 1% NEAA and passage 10–45 cells were utilised for wound repair and migration assay.

Techniques: Marker, Isolation, Immunocytochemistry, Staining

Journal: Journal of Cellular and Molecular Medicine

Article Title: LncRNA‐ATB regulates epithelial‐mesenchymal transition progression in pulmonary fibrosis via sponging miR‐29b‐2‐5p and miR‐34c‐3p

doi: 10.1111/jcmm.16758

Figure Lengend Snippet: TGF‐β1 promotes EMT process and decreases miR‐29b‐2‐5p and miR‐34c‐3p levels. A, Western blot analysis of E‐cadherin, Vimentin, Fibronectin and α‐SMA in A549 cells and BEAS‐2B cells treated with 0, 1, 2 and 5 ng/mL TGF‐β1 for 48 h. B, Western blot analysis of E‐cadherin, Vimentin, Fibronectin and α‐SMA in human primary type II AECs treated with 0 or 5 ng/mL TGF‐β1 for 48 h. C, Wound healing assays were performed to measure the migration ability of A549 cells treated with 0, 1, 2 and 5 ng/mL TGF‐β1 for 48 h. D, Immunofluorescence staining of Vimentin and E‐cadherin in A549 cells for the control and TGF‐β1 (0 or 5 ng/mL) treatment groups. Green represents Vimentin staining; red represents E‐cadherin staining; and blue represents nuclear DNA staining by DAPI. The scale bar is 50 μm. E, qRT‐PCR detection of lncRNA‐ATB expression in 0, 1, 2 and 5 ng/mL TGF‐β1 treated A549 and BEAS‐2B cells for 48 h (mean ± SD, n = 3), * P < .05 difference from untreated cells. F, qRT‐PCR analysis of miR‐29b‐2‐5p and miR‐34c‐3p expression in 0, 1, 2 and 5 ng/mL TGF‐β1 treated A549 and BEAS‐2B cells for 48 h (mean ± SD, n = 3), * P < .05 difference from untreated cells. G, qRT‐PCR detection of lncRNA‐ATB, miR‐29b‐2‐5p and miR‐34c‐3p expression in human primary type II AECs after 0 or 5 ng/mL TGF‐β1 treatment (mean ± SD, n = 3), * P < .05

Article Snippet: Human primary type II alveolar epithelial cells (AECs) were obtained from Procell Biotechnology and cultured in human type II alveolar epithelial cell medium.

Techniques: Western Blot, Migration, Immunofluorescence, Staining, Control, Quantitative RT-PCR, Expressing